Search for dissertations about: "Glycosyl hydrolases"

Showing result 1 - 5 of 6 swedish dissertations containing the words Glycosyl hydrolases.

  2. 1. Thermostable glycosidases and glycosynthases as biocatalysts in green chemistry applications

    Author : Tania Pozzo; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Green chemistry; 3D sturcture; Glycosyl hydrolases; Glycosynthases; Alkyl glucosides; Oligosaccharides; Flavonoids;

    Abstract : The constant need to develop environmental friendly processes using renewable raw materials and to reduce harmful waste production motivates the present investigation focusing on biocatalyst development for enzymatic synthesis, a biotechnological approach in the field of green chemistry. At first, structural functional experiments were made for a β-glucosidase from T. READ MORE

  4. 2. Enzymatic Synthesis of Alkyl Glycosides. New Applications for Glycoside Hydrolases

    Author : David Svensson; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; CGTase; cyclodextrin glycosyltransferase; alkyl glycoside; enzyme stabliity; enzymatic synthesis; enzyme immobilisation; surfactant; glycosylation;

    Abstract : The environmental problems caused by human interventions, such as the consumption of non-renewable resources and the emission of greenhouse gases are well-recognised. Calls have been made for immediate measures to develop more sustainable society worldwide. READ MORE

  5. 3. Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases

    Author : Mathias Wiemann; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; alpha-galactosidase; enzyme engineering; transglycosylation; MALDI-TOF MS; HPLC;

    Abstract : Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently attached to the enzyme, some GHs are able to catalyse synthesis reactions called transglycosylation. READ MORE

  6. 4. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

    Author : Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

    Abstract : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. READ MORE

  7. 5. Hemicelluloses from Agricultural and Forestry Crops Isolation, Characterisation, and Enzymatic Hydrolysis

    Author : Alexandra Andersson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; Physical chemistry; Fysikalisk kemi; enzymology; hydrogels; Plant biochemistry; Växtbiokemi; Proteins; a-galactosidase; b-mannanase; hemicellulase; corn fibre; barley husks; spruce; size-exclusion chromatography; arabinoxylan; microwave irradiation; steam pretreatment; membrane filtration; hemicellulose; galactoglucomannan;

    Abstract : Hemicelluloses are tremendous sources of renewable raw materials, which utilisation have the potential to be highly increased in the future. Hemicelluloses are among the major groups of compounds in plant cell walls, together with cellulose and lignin. These three components are closely associated, making them difficult to separate from each other. READ MORE