Search for dissertations about: "conditioned medium"
Showing result 1 - 5 of 45 swedish dissertations containing the words conditioned medium.
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1. Regulation of productivity in Trichoplusia ni and Spodoptera frugiperda Sf9 serum-free cultures
Abstract : The aim of this work has been to characterize the effects of conditioned medium (CM) on insect cell productivity and physiology in order to get a better understanding about the mechanisms that regulate productivity in serum-free media. Two cell lines have been investigated, Spodoptera frugiperda (Sf9) and Trichoplusia ni (T. ni, BTI-Tn-5B1-4). READ MORE
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2. Physiological effects of conditioned medium and passage number on Spodoptera frugiperda Sf9 serum free cultures
Abstract : The aim of this study was to better understand the role of conditioned medium (CM) in Spodoptera frugiperda Sf9 insect cell proliferation and recombinant protein production using the baculovirus expression system. CM was found to stimulate cell proliferation. READ MORE
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3. Development of a protein-free fed-batch process for NS0 cells: studies on regulation of proliferation
Abstract : The overall objective of this study was to investigate how NS0 cell proliferation is regulated in protein-free media. The hypothesis was that during the adaptation to growth factor-free media, animal cell lines start to produce their own autocrine growth factors to support proliferation, and after some time in a culture the effects of these factors are lost which results in cessation of proliferation. READ MORE
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4. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures
Abstract : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. READ MORE
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5. Optimizing stem cells for reconstructive surgery
Abstract : Fat grafting has become an established method in plastic surgery for treating soft tissue defects. The results for survival of the fat being transplanted is unpredictable and supplementation of the graft with the Stromal Vascular Fraction (SVF) or cultures Adipose tissue-derived stem cells (ASCs) can enhance graft viability. READ MORE