Search for dissertations about: "large scale sequencing"

Showing result 1 - 5 of 108 swedish dissertations containing the words large scale sequencing.

2. 1. Analysis of large-scale metagenomic data

   Author : Fredrik Boulund; Göteborgs universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; metagenomics; DNA analysis; big data; antibiotic resistance; hidden Markov models; high-performance computing; distributed computing; distributed computing;

   Abstract : The topic of this thesis is the analysis of large data sets of DNA sequence data produced from modern high-throughput DNA sequencing machines. Using such machines to sequence the genetic content of a microbial community produces a metagenome. This thesis comprises three research papers, all connected to the study of large metagenomic data sets. READ MORE

4. 2. Genetic Cartography at Massively Parallel Scale

   Author : Johan Dahlberg; Ann-Christine Syvänen; Adam Ameur; Michael C. Zody; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Acute Lymphoblastic Leukemia ALL ; RNA-Sequencing; Bioinformatics; Pooling; Whole Genome Sequencing; Medicinsk genetik; Medical Genetics; Bioinformatics; Bioinformatik;

   Abstract : Massively parallel sequencing (MPS) is revolutionizing genomics. In this work we use, refine, and develop new tools for the discipline.MPS has led to the discovery of multiple novel subtypes in Acute Lymphoblastic Leukemia (ALL). In Study I we screen for fusion genes in 134 pediatric ALL patients, including patients without an assigned subtype. READ MORE

5. 3. Barcoded DNA Sequencing for Parallel Protein Detection

   Author : Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

   Abstract : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. READ MORE

6. 4. Approaches for Distributing Large Scale Bioinformatic Analyses

   Author : Martin Dahlö; Ola Spjuth; Hedi Peterson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; bioinformatics; cloud computing; HPC; high-performance computing; big data; kubernetes; spark; Bioinformatics; Bioinformatik;

   Abstract : Ever since high-throughput DNA sequencing became economically feasible, the amount of biological data has grown exponentially. This has been one of the biggest drivers in introducing high-performance computing (HPC) to the field of biology. READ MORE

7. 5. Molecular Tools for Nucleic Acid Analysis

   Author : Deirdre O'Meara; KTH; []
   Keywords : nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

   Abstract : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. READ MORE