Search for dissertations about: "high ligation"
Showing result 1 - 5 of 87 swedish dissertations containing the words high ligation.
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1. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses
Abstract : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. READ MORE
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2. High Content Analysis of Proteins and Protein Interactions by Proximity Ligation
Abstract : Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. READ MORE
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3. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes
Abstract : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. READ MORE
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4. Visualization of Protein Activity Status in situ Using Proximity Ligation Assays
Abstract : In 2001 the human proteome organization (HUPO) was created with the ambition to identify and characterize all proteins encoded in the human genome according to several criteria; their expression levels in different tissues and under different conditions; the sub-cellular localization; post-translational modifications; interactions, and if possible also the relationship between their structure and function.When the knowledge of different proteins and their potential interactions increases, so does the need for methods able to unravel the nature of molecular processes in cells and organized tissues, and ultimately for clinical use in samples obtained from patients. READ MORE
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5. Proximity Ligation as a Universal Protein Detection Tool
Abstract : Among the great challenges in biology are the precise quantification of specific sets of proteins and analyses of their patterns of interaction on a much larger scale than is possible today.This thesis presents a novel protein detection technique - proximity ligation - and reports the development and application of a nucleic acid amplification technique, RCA. READ MORE